期刊
JOURNAL OF COLLOID AND INTERFACE SCIENCE
卷 556, 期 -, 页码 266-277出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2019.08.010
关键词
Bile salts; Lipid digestion; Interfacial properties; DPPC monolayer; Neutron reflectometry
资金
- EPSRC [EP/L000202/1, EP/R029431/1, EP/P020194/1]
- Biotechnology and Biological Sciences Research Council (BBSRC) through the BBSRC Institute Strategic Programme Food Innovation and Health [BB/R012512/1, BBS/E/F/000PR10345]
- BBSRC [BBS/E/F/00044418, BBS/E/F/000PR10345] Funding Source: UKRI
- EPSRC [EP/R029431/1] Funding Source: UKRI
Hypotheses: Understanding the mechanisms underlying lipolysis is crucial to address the ongoing obesity crisis and associated cardiometabolic disorders. Bile salts (BS), biosurfactants present in the small intestine, play key roles in lipid digestion and absorption. It is hypothesised that their contrasting functionalities - adsorption at oil/water interfaces and shuttling of lipolysis products away from these interfaces - are linked to their structural diversity. We investigate the interfacial films formed by two BS, sodium taurocholate (NaTC) and sodium taurodeoxycholate (NaTDC), differing by the presence or absence of a hydroxyl group on their steroid skeleton. Experiments: Their adsorption behaviour at the air/water interface and interaction with a phospholipid monolayer - used to mimic a fat droplet interface - were assessed by surface pressure measurements and ellipsometry, while interfacial morphologies were characterised in the lateral and perpendicular directions by Brewster angle microscopy, X-ray and neutron reflectometry, and molecular dynamics simulations. Findings: Our results provide a comprehensive molecular-level understanding of the mechanisms governing BS interfacial behaviour. NaTC shows a higher affinity for the air/water and lipid/water interfaces, and may therefore favour enzyme adsorption, whereas NaTDC exhibits a higher propensity for desorption from these interfaces, and may thus more effectively displace hydrolysis products from the interface, through dynamic exchange. (C) 2019 Elsevier Inc. All rights reserved.
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