期刊
JOURNAL OF CLINICAL LABORATORY ANALYSIS
卷 34, 期 4, 页码 -出版社
WILEY
DOI: 10.1002/jcla.23131
关键词
biomarker; gastric cancer; gastric juice; piR-1245; PIWI-interacting RNA
资金
- China Postdoctoral Science Foundation Funding Source: Medline
- National Natural Science Foundation of China [2019T120406, 2017M620197, 81570186] Funding Source: Medline
- The talents program of Jiangsu Cancer Hospital [YC201808] Funding Source: Medline
- the Research Office of Jiangsu Cancer Hospita [ZK201401] Funding Source: Medline
- Jiangsu Provincial Medical Youth Talent [QNRC2016644] Funding Source: Medline
- Jiangsu Government Scholarship for Overseas Studies [JS-2017-123] Funding Source: Medline
- Human Resource Summit Grant of Jiangsu Province [WSN-53] Funding Source: Medline
- Southeast university-Nanjing medical university cooperation program [2017DN42] Funding Source: Medline
Background Emerging reports demonstrated that PIWI-interacting RNAs (piRNAs) played an indispensable role in tumorigenesis. However, it still remains elusive whether piR-1245 in gastric juice specific in stomach could be employed as a biomarker for gastric cancer (GC). The present work is aiming at exploring the possibility of piR-1245 in gastric juice as a potential marker to judge for diagnosis and prognosis of gastric cancer. Methods Gastric juice was collected from 66 GC patients and 66 healthy individuals. Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) was employed to measure the levels of piR-1245 expression. Then, the pattern of piR-1245 expression in gastric juice was determined between GC patients and healthy individuals. A receiver operating characteristic (ROC) curve was constructed for distinguishing GC from healthy individuals. Results Gastric juice piR-1245 levels in GC were higher than those of controls (P < .0001). The value of area under ROC (AUC) was 0.885 (sensitivity, 90.9%; specificity, 74.2%; 95% confidence interval, 0.8286 to 0.9414). High gastric juice piR-1245 expression was signally correlated with tumor size (P = .013) and TNM stage (P = .001). GC patients with high piR-1245 expression in gastric juice exerted a poorer overall survival (OS) (P = .0152) and progression-free survival (PFS) (P = .013). COX regression analysis verified that gastric juice piR-1245 expression was an independent prognostic risk variable for OS (P < .05). Conclusions The current study suggested that piR-1245 in gastric juice had the potential to be a useful biomarker for GC detection and prognosis prediction.
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