期刊
BIOMICROFLUIDICS
卷 10, 期 6, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.4968033
关键词
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资金
- NIH/NIAID
- NSF [4R33AI100229, 1R01AI116989-01, CBET-1159453, CBET-1159423]
- W. M. Keck Center for Nanoscale Optofluidics at the University of California at Santa Cruz
- Eugene Cota-Robles Fellowship
- RMI Graduate Research Fellowship
We have developed an optofluidic analysis system that processes biomolecular samples starting from whole blood and then analyzes and identifies multiple targets on a silicon-based molecular detection platform. We demonstrate blood filtration, sample extraction, target enrichment, and fluorescent labeling using programmable microfluidic circuits. We detect and identify multiple targets using a spectral multiplexing technique based on wavelength-dependent multi-spot excitation on an antiresonant reflecting optical waveguide chip. Specifically, we extract two types of melanoma biomarkers, mutated cell-free nucleic acids -BRAFV600E and NRAS, from whole blood. We detect and identify these two targets simultaneously using the spectral multiplexing approach with up to a 96% success rate. These results point the way toward a full front-to-back chip-based optofluidic compact system for high-performance analysis of complex biological samples. Published by AIP Publishing.
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