The spindle pole body of Aspergillus nidulans is asymmetrical and contains changing numbers of γ-tubulin complexes

Centrosomes are important microtubule-organizing centers (MTOCs) in animal cells. In addition, non-centrosomal MTOCs (ncMTOCs) are found in many cell types. Their composition and structure are only poorly understood. Here, we analyzed nuclear MTOCs (spindle-pole bodies, SPBs) and septal MTOCs in Aspergillus nidulans. They both contain gamma-tubulin along with members of the family of gamma-tubulin complex proteins (GCPs). Our data suggest that SPBs consist of gamma-tubulin small complexes (gamma-TuSCs) at the outer plaque, and larger gamma-tubulin ring complexes (gamma-TuRC) at the inner plaque. We show that the MztA protein, an ortholog of the human MOZART protein (also known as MZT1), interacted with the inner plaque receptor PcpA (the homolog of fission yeast Pcp1) at SPBs, while no interaction nor colocalization was detected between MztA and the outer plaque receptor ApsB (fission yeast Mto1). Septal MTOCs consist of gamma-TuRCs including MztA but are anchored through AspB and Spa18 (fission yeast Mto2). MztA is not essential for viability, although abnormal spindles were observed frequently in cells lacking MztA. Quantitative PALM imaging revealed unexpected dynamics of the protein composition of SPBs, with changing numbers of gamma-tubulin complexes over time during interphase and constant numbers during mitosis. This article has an associated First Person interview with the first author of the paper.