期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 108, 期 2, 页码 316-326出版社
WILEY
DOI: 10.1002/jbm.a.36818
关键词
cell-material interaction; osteogenesis; phosphate glass fibers; Runx2; signaling pathway
资金
- China Postdoctoral Science Foundation [2017M613196]
- Fundamental Research Funds for the Central Universities [3102017OQD041, 3102019ghxm012]
- National Natural Science Foundation of China [31800802, 81601913, 81700784]
- Nature Science Basic Research Plan in Shannxi Province of China [2018JQ3049]
- Shaanxi Provincial Key RD Program [2018KW-031]
Cell-material interactions and compatibility are important aspects of bioactive materials for bone tissue engineering. Phosphate glass fiber (PGF) is an attractive inorganic filler with fibrous structure and tunable composition, which has been widely investigated as a bioactive filler for bone repair applications. However, the interaction of osteoblasts with PGFs has not been widely investigated to elucidate the osteogenic mechanism of PGFs. In this study, different concentrations of short PGFs with interlaced oriented topography were cocultured with MC3T3-E1 cells for different periods, and the synergistic effects of fiber topography and ionic product of PGFs on osteoblast responses including cell adhesion, spreading, proliferation, and osteogenic differentiation were investigated. It was found that osteoblasts were more prone to adhere on PGFs through Vinculin protein, leading to enhanced cell proliferation with polygonal cell shape and spreading cellular actin filaments. In addition, osteoblasts incubated on PGF meshes showed enhanced alkaline phosphatase activity, extracellular matrix mineralization, and increased expression of osteogenesis-related marker genes, which could be attributed to the Wnt/beta-catenin/Runx2 signaling pathway. This study elucidated the possible mechanism of PGF on triggering specific osteoblast behavior, which would be highly beneficial for designing PGF-based bone graft substitutes with excellent osteogenic functions.
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