期刊
JOURNAL OF APPLIED MICROBIOLOGY
卷 128, 期 2, 页码 414-425出版社
OXFORD UNIV PRESS
DOI: 10.1111/jam.14493
关键词
antimicrobial peptide (AMP); C; albicans; clinical isolates; crotalicidin peptides; Crypctococcus neoformans; drug-resistant yeasts
资金
- Coordination for the Improvement of Higher Education Personnel (CAPES)
- Ministry of Education and Culture (MEC)
- Federal Government of Brazil
- FUNCAP
- Spanish Ministry of Economy and Competitiveness (MINECO) [AGL2014-52395-C2, AGL2017-84097-C2-2-R]
- 'Maria de Maeztu' Program for Units of Excellence in RD [MDM-2014-0370]
Aims Crotalicidin (Ctn), a cathelicidin-related antimicrobial peptide from the South American rattlesnake venom gland, and its C-terminal Ctn[15-34] fragment, have exhibited important activities against micro-organisms, trypanosomatid protozoa and certain lines of tumour cells. Herein, the activity against clinical strains of fluconazole-resistant Candida albicans and of amphotericin B and fluconazole-resistant Cryptococcus neoformans was investigated. Methods and Results Microdilution and luminescent cell viability tests were used to evaluate and compare the susceptibility of pathogenic yeasts to these peptides. The time-kill curves of the most active Ctn[15-34] alone or in combination with fluconazole against drug-resistant yeasts were determined. Concomitantly, the fungicidal and/or fungistatic effects of Ctn[15-34] were visualized by the spotting test. The peptides were active against all strains, including those resistant to antifungal agents. The association of fluconazole with both Ctn and Ctn[15-34], although not synergic, was additive. In contrast, such pattern was not observed for C. neoformans. Conclusions Overall, Ctn and Ctn[15-34] are potential antifungal leads displaying anti-yeast activities against clinical isolates endowed with drug resistance mechanisms. Significance and Impact of the Study The effective peptide activity against resistant strains of pathogenic yeasts demonstrates that crotalicidin-derived peptides are promising templates to develop new antifungal pharmaceuticals.
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