期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 48, 页码 13344-13352出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b05441
关键词
anthranilic diamide derivatives; hydrazone; trifluoromethylpyridine synthesis; antiviral activity; interactions
资金
- National Natural Science Foundation of China [21762012, 21562012]
- S&T Planning Project of Guizhou Province [[2017]1402, [2017]5788]
- Guizhou Province Governor Foundation for Outstanding Talents [2015-15#]
A series of novel anthranilic diamide derivatives (5a-5ab) containing moieties of trifluoromethylpyridine and hydrazone was designed and synthesized. The synthesized compounds were evaluated in vivo for their activities against tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV). Most of the synthesized compounds displayed good to excellent antiviral activities. The compounds 5i, 5k, 5s, 5w, 5x, and 5z had the curative activity over 65% against TMV at the concentration of 500 mu g/mL, which were significantly higher than those of ningnanmycin (55.0%) and ribavirin (37.9%). Notably, the curative activity of compound Si was up to 79.5%, with the EC50 value of 75.9 mu g/mL, whereas the EC50 value of ningnanmycin was 362.4 mu g/mL. The pot experiments also further demonstrated the significantly curative effect of Si. Meanwhile, compounds 5h, 5p and Sx displayed more protective activities on TMV than that of ningnanmycin. Moreover, compounds 5a, 5e, 5f, and 5i showed inactivation activity similar to ningnanmycin at 500 mu g/mL, and the EC50 value of Se (41.5 mu g/mL) was lower than ningnanmycin (50.0 mu g/mL). The findings of transmission electron microscopic (TEM) indicated that the synthesized compounds exhibited strong and significant binding affinity to TMV coat protein (CP) and could obstruct the self-assembly and increment of TMV particles. Microscale thermophoresis (MST) studies on TMV-CP and CMV CP revealed that some of the active compounds, particularly 5i, exhibited a strong binding capability to TMV-CP or CMV-CP. This study revealed that anthranilic diamide derivatives containing moieties of trifluoromethylpyridine and hydrazone could be used as novel antiviral agents for controlling the plant viruses.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据