期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 68, 期 4, 页码 1015-1021出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b05218
关键词
(2S)-naringenin; p-coumaric acid; shikimate pathway; chalcone synthase; yeast
资金
- National Key Research and Development Program of China [2017YFC1600403]
- National Science Fund for Excellent Young Scholars [21822806]
- National Natural Science Foundation of China [31670095, 31770097]
- China Postdoctoral Science Foundation [2019 M651701]
- Fundamental Research Funds for the Central Universities [JUSRP51701A]
- National First-class Discipline Program of Light Industry Technology and Engineering [LITE2018-08]
- Distinguished Professor Project of Jiangsu Province
(2S)-Naringenin, a (2S)-flavanone, is widely used in the food, chemical, and pharmaceutical industries because of its diverse physiological activities. The production of (2S)-naringenin in microorganisms provides an ideal source that reduces the cost of the flavonoid. To achieve efficient production of (2S)-naringenin in Saccharomyces cerevisiae (S. cerevisiae), we constructed a biosynthetic pathway from p-coumaric acid, a cost-effective and more efficient precursor. The (2S)-naringenin synthesis pathway genes were integrated into the yeast genome to obtain a (2S)-naringenin production strain. After gene dosage experiments, the genes negatively regulating the shikimate pathway and inefficient chalcone synthase activity were verified as factors limiting (2S)-naringenin biosynthesis. With fed-batch process optimization of the engineered strain, the titer of (2S)-naringenin reached 648.63 mg/L from 2.5 g/L p-coumaric acid. Our results indicate that the constitutive production of (2S)-naringenin from p-coumaric acid in S. cerevisiae is highly promising.
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