期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 46, 页码 12816-12823出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b04354
关键词
pendimethalin; dinitroaniline herbicides; Bacillus subtilis Y3; nitroreductase; catalytic efficiency
资金
- National Natural Science Foundation of China [31760031, 31600080, 31560031]
- National Science and Technology Major Project [2018ZX0800907B-002]
- Natural Science Foundation of Jiangxi Province [20171BAB214002]
- Educational Committee of Jiangxi Province [GJJ170232]
Microbial degradation plays a major role in the dissipation of pendimethalin, and nitroreduction is an initial and detoxicating step. Previously, a pendimethalin nitroreductase, PNR, was identified in Bacillus subtilis Y3. Here, another pendimethalin nitroreductase from strain Y3, LNR, was identified. LNR shares only 40% identity with PNR and reduces the aromatic ring C-6 nitro group of pendimethalin and both nitro groups of trifluralin, butralin, and oryzalin. The catalytic activities against the four dinitroanilines were much higher for LNR than for PNR. lnr deletion significantly reduced the pendimethalin-reduction activity (60% activity loss), while pnr deletion led to only 30% activity loss, indicating that both LNR and PNR were involved in pendimethalin nitroreduction in strain Y3; however, LNR played the major role. This study facilitates the elucidation of pendimethalin catabolism and provides degrading enzyme resources for the removal of dinitroaniline herbicide residues in environment and agricultural products.
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