期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 68, 期 39, 页码 10951-10961出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b05714
关键词
Fra a 1; Fragaria x ananassa; allergen; PR-10; IgE binding; 3D-structure; hypoallergenic mutants
资金
- MEC, Spain [BIO2013-44199-R]
- Ramon y Cajal program (MINECO, Universidad de Malaga, Spain) [RYC-2017-22323]
- European Community's Seventh Framework Programme H2020 under iNEXT (H2020 Grant) [653706]
- MINECO, Spain [BIO2017-84548-R]
- Instituto de Salud Carlos III (ISCIII) [RD16/006/003]
- [BES-2014-068723]
- MRC [MC_U105192715] Funding Source: UKRI
Although strawberries are highly appreciated fruits, their intake can induce allergic reactions in atopic patients. These reactions can be due to the patient's previous sensitization to the major birch pollen allergen Bet v 1, by which IgE generated in response to Bet v 1 cross-reacts with the structurally related strawberry Fra a 1 protein family. Fra a 1.02 is the most expressed paralog in ripe strawberries and is highly allergenic. To better understand the molecular mechanisms regulating this allergic response, we have determined the three-dimensional structure of Fra a 1.02 and four site-directed mutants that were designed based on their positions in potential epitopes. Fra a 1.02 and mutants conform to the START fold. We show that the cross-reactivity of all the mutant variants to IgE from patients allergic to Bet v 1 was significantly reduced without altering the conserved structural fold, so that they could potentially be used as hypoallergenic Fra a 1 variants for the generation of vaccines against strawberry allergy in atopic patients.
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