期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 41, 页码 11481-11488出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b04933
关键词
mycotoxin; aflatoxins; dark tea; cleaning method; LC-MS/MS
资金
- National Key R&D Program of China [2017YFC1600902]
- National Natural Science Foundation of China [31671949]
- Anhui Natural Science Foundation [1608085J08]
- Foundation from Shanghai Key Laboratory of Bio-Energy Crops, Shanghai University
Dry tea matrix contains an abundance of caffeine and polyphenols which are different from the food matrix (e.g., protein, lipid, and carbohydrates), and only a few studies have tried aflatoxins determination with tea samples. Here, a specific, accurate, and sensitive method was developed and validated for the simultaneous determination of aflatoxin B1, B2, G1, and G2 in dark teas. Aflatoxins were extracted by acetonitrile/water, press-filtered, and cleaned by multifunctional purification column (MFC) and immunoaffinity column (IAC) in tandem. The cleaned extract was analyzed by liquid chromatography tandem mass spectrometry. The matrix interference was effectively reduced by MFC-IAC cleaning method. Recoveries at the spiking concentrations of 5-60 mu g/kg ranged from 77.5 to 93%, with relative standard deviations <11.0%. The correlation coefficients of aflatoxins standard were >0.998. The limits of detection were 0.024-0.21 mu g/kg and the limits of quantification were 0.08-0.74 mu g/kg. The intra- and interday accuracy ranged from 74 to 87%, and the intra- and interday precisions ranged from 0.4 to 3.1%. After the method validation, the aflatoxins contaminations in 100 collected dark teas were detected, and the results were compared with those of other methods.
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