期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 45, 页码 12528-12537出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b06533
关键词
broccoli; glucosinolate; sulforaphane; isothiocyanate; hydrolysis
资金
- National Key Research and Development Program of China [2017YFE0114500, 2017YFD0101805]
- National Natural Science Foundation of China [31200230]
- Science and Technology Department of Zhejiang Province [2016C02051-1]
- Bureau of Agriculture and Rural Affairs of Taizhou City [TZ2018015]
- Ningbo Science and Technology Program [2015C110008]
- ZAAS (Al)
- European Union [774244]
Seeds of 32 pure lines and 6 commercial broccoli cultivars were used to investigate variation in glucosinolates and their breakdown products. The aliphatic glucosinolate content was 54.5-218.7 mu mol/g fresh weight, accounting for >90% of the total glucosinolates. The major glucosinolates found were glucoraphanin and glucoerucin in 27 samples and progoitrin in 7 samples. A gas chromatography-flame ionization detector (GC-FID) method was used to identify glucosinolate breakdown products; nine products were directly determined using standards. Using Arabidopsis thaliana lines myb28myb29 and Landsberg erecta to hydrolyze each reference glucosinolate, seven products were tentatively identified. 4-(Methylsulfinyl)butyl isothiocyanate and 5-(methylsulfinyl)pentanenitrile contents were 2.6-91.1 mu mol/g fresh weight and 0-35.4 mu mol/g fresh weight, respectively, with epithionitriles being more common than nitriles in accessions rich in alkenyl glucosinolate. Additionally, (S)-5-vinyl-1,3-oxazolidine-2-thione was detected in accessions rich in progoitrin. Specific lines with altered glucosinolate profiles and breakdown products were obtained and discussed according to the putative glucosinolate metabolism pathway.
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