期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 42, 页码 11758-11768出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b05144
关键词
Pichia caribbica; PcCRG1; degradation; patulin; biocontrol
资金
- National Natural Science Foundation of China [31772037]
- Natural Science Foundation of the Jiangsu Higher Education Institutions of China [18KJB550002]
Patulin contamination not only is a menace to human health but also causes serious environmental problems worldwide due to the synthetic fungicides that are used to control it. This study focused on investigating the patulin degradation mechanism in Pichia caribbica at the molecular level. According to the results, P. caribbica (2 x 10(6) cells/mL) was able to degrade patulin from 20 mu g/mL to an undetectable level in 72 h. The RNA-seq data showed patulin-induced oxidative stress and responses in P. caribbica. The deletion of PcCRG1 led to a significant decrease in patulin degradation by P. caribbica, whereas the overexpression of PcCRG1 accelerated the degradation of patulin. The study identified that PcCRG1 protein had the ability to degrade patulin in vitro. Overall, we demonstrated that the patulin degradation process in P. caribbica was more than one way; PcCRG1 was an S-adenosylmethionine-dependent methyltransferase and played an important role in the patulin degradation process in P. caribbica.
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