期刊
BIOMEDICINE & PHARMACOTHERAPY
卷 81, 期 -, 页码 63-70出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biopha.2016.03.040
关键词
microRNA; miR-128a; Pre-eclampsia; Bax; Apoptosis; Mitochondria
资金
- Natural Science Foundation of Jiangsu Province, China [BK20131076]
- National Natural Science Foundation of China [81571458, 81501278]
- Nanjing Medical Technology Development Project [JQX1501]
- Nanjing health talent project
- Jiangsu maternal and child health key project
Introduction: Pre-eclampsia (PE) can endanger the survival of the mother and fetus. Currently, the pathogenesis of PE is not completely understood and no fundamental therapeutics are available. The present study was performed to determine the function of miR-128a in HTR-8/SVneo trophoblast cells and to ascertain its underlying role in the pathogenesis of PE. Methods: We investigated the function of miR-128a in HTR-8/SVneo cells by overexpressing. We analyzed the apoptosis of HTR-8/SVneo cells by performing apoptosis assays and measured the loss of mitochondrial membrane potential (Delta ym), the generation of reactive oxygen species (ROS) and caspase activity. In addition, miR-128a target genes were predicted. Results: Using computer-based programs, we identified Bax as a direct target of miR-128a. In the apoptosis assays of HTR-8/SVneo cells, miR-128a decreased the Delta psi m, depleted ATP levels and increased ROS generation, cytochrome c release as well as caspase activation. Further studies showed that miR-128a induced the apoptosis of HTR-8/SVneo cells by down-regulating Bax through the mitochondrial apoptosis pathway. Conclusions: miR-128a is an up-regulated miRNA in patient with PE. Our study demonstrated that the miR-128a-induced apoptosis of HTR-8/SVneo cells may contribute to PE and miR-128a may be a novel potential therapeutic target for PE. (C) 2016 Elsevier Masson SAS. All rights reserved.
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