Enhancement of orofacial antinociceptive effect of carvacrol, a monoterpene present in oregano and thyme oils, by β-cyclodextrin inclusion complex in mice

Orofacial pain is associated with diagnosis of chronic pain of head, face, mouth, neck and all the intraoral structures. Carvacrol, a naturally occurring isoprenoid with diverse class of biological activities including anti-inflammatory, analgesic, antitumor and antioxidant properties. Now, the antinociceptive effect was studied in mice pretreatment with carvacrol (CARV) and beta-cyclodextrin complex containing carvacrol (CARV-beta CD) in formalin-, capsaicin-, and glutamate-induced orofacial nociception. Mice were pretreated with vehicle (0.9% Nacl, p.o.), CARV (10 and 20 mg/kg, p.o.), CARV-beta CD (10 and 20 mg/kg, p.o.) or MOR (10 mg/kg, i.p.) before the nociceptive behavior induced by subcutaneous injections (s.c.) of formalin (20 mu l, 2%), capsaicin (20 mu l, 2.5 mu g) or glutamate (20 mu l, 25 mu M) into the upper lip respectively. The interference on motor coordination was determined using rotarod and grip strength meter apparatus. CARV-beta CD reduced the nociceptive during the two phases of the formalin test, whereas CARV did not produced the reduction in face-rubbing behavior in the initial phase. CARV-beta CD (20 mg/kg, p.o.) produced 49.3% behavior pain while CARV alone at 20 mg/kg, p.o, produced 28.7% of analgesic inhibition in the second phase of formalin test. CARV, CARV-beta CD and Morphine (MOR) showed a significant reduction against nociception caused by capsaicin or glutamate injection. Thus the encapsulation of carvacrol in beta-cyclodextrin can acts as a considerable therapeutic agent with pharmacological interest for the orofacial pain management. (C) 2016 Elsevier Masson SAS. All rights reserved.