期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 143, 期 -, 页码 724-731出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.10.002
关键词
Silver nanoparticles; Trimethylchitosan; Anti-Candida; Microdilution inhibition assay; Zebrafish egg biofilm assay
资金
- Ministry of Science and Technology of the Republic of China [MOST 107-2623-E-016-001-D, MOST 108-2623-E-016-003-D, MOST 108-2623-E-011-002-D]
- Chang Gung Memorial Hospital of the Republic of China [CMRPG3H0121]
- National Defense Medical Center of the Republic of China [MAB-107-045, IPM-107-G5-6, IPM-107-G6-1]
Herein, positively surface-charged silver nanopartides (AgNPs) capped with trimethylchitosan nitrate (TMCN) were synthesized using an environmentally friendly method. Nano-sized TMCN-AgNPs (similar to 80 nm) with high zeta potential (>30 mV) provide sufficient static repulsion to stabilize colloid AgNPs in aqueous solutions without aggregation for >3 months. In in vitro cell cycle assays, TMCN-AgNPs showed low cytotoxicity towards L929 cells. A microdilution inhibition assay demonstrated the antifungal potential of TMCN-AgNPs, with a minimum inhibitory concentration of 0.06 mM against Candida tropicalis ATCC 750, and 0.46 mM against both Candida albicans ATCC 76615 and Candida glabrata ATCC 15545. Moreover, the addition of TMCN-AgNPs at 0.23 mM significantly reduced biofilm formation in 96-well plates with C albicans and C tropicalis. Importantly, when zebrafish eggs were infected with Candida cells, 0.23 mM TMCN-AgNPs greatly diminished the amount of biofilm on eggs and rescued the survival of embryos by up to 70%. (C) 2019 Elsevier B.V. All rights reserved.
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