期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 151, 期 -, 页码 1163-1172出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.10.160
关键词
trans-polydatin and trans-resveratrol; G-quadruplex; Melanoma cells
资金
- AIRC [17037, 17585]
- Gaussian Inc. (Wallingford, CT)
- GLURES S.r.l (Venezia, Italy) [EP1292319A2]
Among polyphenols, trans-resveratrol (tRES) and trans-polydatin (tPD) exert multiple biological effects, particularly antioxidant and antiproliferative. In this work, we have investigated the interaction of tPD with three cancer-related DNA sequences able to form G-quadruplex (G4) structures, as well as with a model duplex, and compared its behaviour with tRES. Interestingly, fluorescence analysis evidenced the ability of tPD to bind all the studied DNA systems, similarly to tRES, with tRES displaying a higher ability to discriminate G4 over duplex with respect to tPD. However, neither tRES nor tPD produced significant conformational changes of the analyzed DNA upon binding, as determined by CD-titration analysis. Computational analysis and biological data confirmed the biophysical results: indeed, molecular docking evidenced the stronger interaction of tRES with the promoter of c-myc oncogene, and immunoblotting assays revealed a reduction of c-myc expression, more effective for tRES than tPD. Furthermore, in vitro assays on melanoma cells proved that tPD was able to significantly reduce telo-merase activity, and inhibit cell proliferation, with tRES producing higher effects than tPD. (C) 2019 Elsevier B.V. All rights reserved.
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