期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 153, 期 -, 页码 1319-1327出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.10.268
关键词
Zein film; Atmospheric cold plasma; Surface properties; Cell culture
资金
- National Natural Science Foundation for Youth of China [31901607]
The cell adhesion and proliferation of zein-based biomaterials in cell culture application are limited by the strong hydrophobic surface with low surface energy. In this study, atmospheric cold plasma (ACP) was developed as a modification approach in enhancing the surface hydrophilic and cytocompatibility of zein films. The results indicated that water contact angles decreased from 72.85 degrees (untreated) to 47.43 degrees under the voltage of 100 V. The improvement of the surface free energy (SFE) was mainly attributed to the polar component rather than dispersive component. X-ray photoelectron spectroscopy (XPS) results indicated the change of surface physicochemical properties was mainly due to the partially transformation of long-chain aliphatic hydrocarbons (C-H and/or C-C) to oxygen- and nitrogen-containing bonds (C=O, C-O and C-N). Furthermore, the content of secondary structure demonstrated beta-turn and alpha-helix were transformed into beta-sheet and random coil after ACP treatment. Combining with the cell experiment results, plasma treatment could significantly improve the adhesion rate and proliferation activity of C2C12 cells on zein films. With better cytocompatibility, the potentials of zein in tissue engineering scaffold could be readily exploited. (C) 2019 Elsevier B.V. All rights reserved.
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