期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 145, 期 -, 页码 1080-1090出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.09.201
关键词
Rosa laevigata Michx; Polysaccharide; Structural characterization; RAW264.7 cells; Immunomodulatory activity
资金
- National Natural Science Foundation of China [31270636, 21676105]
A novel acid polysaccharide (PPRLMF-2) with the M-w of 137,123 Da and a triple-helix conformation was first isolated from the pulp of Rosa laevigata Michx fruit. Structural characterization showed that PPRLMF-2 consisted of rhamnose (7.6%), arabinose (26.5%), xylose (3.5%), mannose (0.9%), glucose (5.7%), galactose (31.9%) and galacturonic acid (23.9%). The methylation and NMR (1 D and 2D) analysis revealed that PPRLMF-2 contained 16 types of glycosidic linkages. The immunomodulatory activity assays indicated that PPRLMF-2 could significantly enhance phagocytosis, the secretion and mRNA expression of cytokines in RAW 264.7 cells. Additionally, SR, GR, TLR-2, and TLR-4 were the main pattern recognition receptors (PRRs) of PPRLMF-2 to upregulate the p-ERK, p-JNK, p-p38, and p-p65. These results suggested that PPRLMF-2 could recognize the PRRs of the macrophages to enhance the immunomodulatory activity via activation of the MAPK5 and NF-kappa B signaling pathways. This study provides important implications of PPRLMF-2 as an attractive immunomodulatory functional food. (C) 2019 Elsevier B.V. All rights reserved.
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