期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 155, 期 -, 页码 951-960出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.11.054
关键词
Diatoms; Polysaccharides; Semi-continuous system
资金
- Ministry of Science and Technology, Taiwan [MOST 105-2221-E-166-007, NSC 100-2321-B-005-004-MY3, NSC 103-2321-B-005-018, MOST 106-2218-E-005-018]
An efficient process has been developed for bioactive polysaccharide production and purification from a local diatom isolate, Halamphora sp. AQ4. First, a semi-continuous system with fixed harvesting frequency was employed to cultivate AQ4 for the production of cell mass and polysaccharides for more than 285 days with a high yield of biomass. Six cultivation sets are performed according to different harvesting volumes per 3 days with or withoutNa(2)CO(3) supplement. The addition of Na2CO3 increases both cellmass and polysaccharide production. Furthermore, three different sulfated polysaccharides (PK1 similar to PK3) were purified fromthe freshly-grown AQ4 diatoms following anion-exchange chromatography. Among them, polysaccharide PK3 not only has a high content of fucose and uronic acid, but also has a strong activity to stimulate murine macrophage cells and increase their phagocytosis rate up to 170%. This study demonstrates that diatom AQ4 is an important bioresource for the production of bioactive polysaccharides. (C) 2019 Published by Elsevier B.V.
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