期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 156, 期 -, 页码 1455-1463出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.11.190
关键词
Chitosaii-coated liposome; N-acetylcysteine; Lung drug delivery
资金
- University of Tehran and Mashhad University of Medical Sciences
The purpose of the present investigation was to formulate NAC (N-acetylcysteine)-loaded chitosan (CH)-coated liposome aiming at obtaining an effective formulation able to ensure a prolonged and controlled release of NAC to the lung by inhalation. Empty liposomes [(DPPG/Chol/DPPG with different molar percentages of DPPG) (0, 1. 2.5, 5 )] were prepared and coated with CH at different CH/Lipid ratio (0.5, 1, 1.5,2,2.5, W/W) to reach optimum coating of CH. TEM and SEM indicated that morphology of CH-coated and -uncoated liposomes were spherical. ITIR analysis indicated attachment of CH on liposome surface. The drug release experiment in the simulated lung fluid showed that the CH-uncoated and -coated liposomes released 51% and 38% of NAC during 9 h, respectively. The results showed that coating of liposome with CH resulted in the prolonged release of NAC from CH-coated liposome. The results of flow cytometry indicated the effective uptake of CH-coated liposome compared with the CH-uncoated liposome in epithelial cells. In vivo experiment indicated good deposition and retention of CH-coated liposome in lung in comparison with CH-uncoated liposome. The results of the present study demonstrated that CH-coated liposome may represent a promising carrier for the delivery of NAC to the lungs by inhalation therapy. (C) 2019 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据