期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 138, 期 -, 页码 511-518出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.07.117
关键词
NMR; Chemical structure; Anticancer activity
资金
- Earmarked Fund for China Agriculture Research System [CARS-26]
- Guangdong Province Science and Technology Plan Project [2018B020202009]
- Guangdong Academy of Agricultural Sciences [201803XX]
- Institute of Fruit Research, Guangdong Academy of Agricultural Sciences [201901]
Citrus grandis 'Tomentosa' (CGT) which is a region-famous medicinal and edible plant contains plentiful bioactive polysaccharides, however, its chemical structures and specific bioactivities still need to be further explored. In the present study, an acidic polysaccharide (CGTP-AP) was extracted and purified from the pulps of CGT. The structure elucidation and anti-cancer activity of CGTP-AP were investigated. Structure characterization indicated that CGTP-AP was a homogeneous heteropolysaccharide composed of arabinose, galactose and galacturonic acid in a molar ratio of 2.45:1:2.77, with an average molecular weight of 2721.68 kDa. Partial acid hydrolysis, methylation and NMR spectrometry revealed that the backbone of CGTP-AP mainly composed of (1 -> 4)-alpha-D-galacturonan, while the branch principally consisted of (1 -> 5)-alpha-L-Araf. In addition, CGTP-AP exhibited effective anti-proliferation against colon cancer cells LOVO and SW620 cells in dose- and time-dependent manners, with IC50 values of 5.55, 4.35 and 3.52 mg mL(-1) after 24, 48 and 72 h, and 5.33, 3.63 and 2.97 mg mL(-1) after 24, 48 and 72 h, respectively. This study indicated that CGTP-AP might be utilized as a promising food supplement for the patients of colon disorders. (C) 2019 Elsevier B.V. All rights reserved.
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