期刊
INFECTION AND IMMUNITY
卷 88, 期 1, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.00541-19
关键词
Chlamydia; NK cells; dendritic cells; exosomes
资金
- Deutsche Forschungsgemeinschaft [SPP1580]
Dendritic cells (DCs) and natural killer (NK) cells are critically involved in the early response against various bacterial microbes. Functional activation of infected DCs and NK cell-mediated gamma interferon (IFN-gamma) secretion essentially contribute to the protective immunity against Ch/amydia. How DCs and NK cells cooperate during the antichlamydial response is not fully understood. Therefore, in the present study, we investigated the functional interplay between Chlamydia-infected DCs and NK cells. Our biochemical and cell biological experiments show that Chla-mydia psittaci-infected DCs display enhanced exosome release. We find that such extracellular vesicles (referred to as dexosomes) do not contain infectious bacterial material but strongly induce IFN-gamma production by NK cells. This directly affects C. psittaci growth in infected target cells. Furthermore, NK cell-released IFN-gamma in cooperation with tumor necrosis factor alpha (TNF-alpha) and/or dexosomes augments apoptosis of both noninfected and infected epithelial cells. Thus, the combined effect of dexosomes and proinflammatory cytokines restricts C. psittaci growth and attenuates bacterial subversion of apoptotic host cell death. In conclusion, this provides new insights into the functional cooperation between DCs, dexosomes, and NK cells in the early steps of antichlamydial defense.
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