期刊
BIOMATERIALS
卷 83, 期 -, 页码 233-248出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2015.11.059
关键词
Neurotrophin-3; Fibroin; Gelatin sponge scaffold; Controlled artificial release system; Transplantation; Spinal cord injury
资金
- Chinese National Natural Science Foundation [81330028, U1301223, 31200737]
- National 863 Project [2013AA020106]
- Foundation of the Education Ministry of China [201300193035]
Persistent neurotrophic factor delivery is crucial to create a microenvironment for cell survival and nerve regeneration in spinal cord injury (SCI). This study aimed to develop a NT-3/fibroin coated gelatin sponge scaffold (NF-GS) as a novel controlled artificial release therapy for SCI. In vitro, bone marrow -derived mesenchymal stem cells (MSCs) were planted into the NF -GS and release test showed that NF -GS was capable to generate a sustainable NT-3 release up to 28 days. MSCs in NF -GS had high cell activity with excellent cell distribution and phenotype. Then, the NF -GS was transplanted into the injury site of spinal cord of rat and canine in vivo, which exhibited strong biocompatibility during post -transplantation period. Four weeks following transplantation, the concentration of NT-3 was much higher than that in control groups. Cavity areas in the injury/graft site were significantly reduced due to tissue regeneration and axonal extensions associated with myelin sheath through the glial scar into the NF -GS. Additionally, the NF -GS decreased the inflammation by reducing the CD68 positive cells and TNF-alpha. A striking feature was the occurrence of some cells and myelin -like structure that appeared to traverse the NF -GS. The present results demonstrate that the NF -GS has the property to control the release of NT-3 from the NT-3/fibroin complex thus facilitating regeneration of injured spinal cord. (C) 2015 Elsevier Ltd. All rights reserved.
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