期刊
BIOMATERIALS
卷 84, 期 -, 页码 76-85出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2016.01.030
关键词
3D tumor model; Tumor microenvironment; Preclinical drug testing; Oncolytic adenovirus
资金
- Cancer Prevention Research Institute of Texas [RP110553 P1]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1055942] Funding Source: National Science Foundation
Interactions between malignant and stromal cells and the 3D spatial architecture of the tumor both substantially modify tumor behavior, including the responses to small molecule drugs and biological therapies. Conventional 2D culture systems cannot replicate this complexity. To overcome these limitations and more accurately model solid tumors, we developed a highly versatile 3D PEG-fibrin hydrogel model of human lung adenocarcinoma. Our model relevantly recapitulates the effect of oncolytic adenovirus; tumor responses in this setting nearly reproduce those observed in vivo. We have also validated the use of this model for complex, long-term, 3D cultures of cancer cells and their stroma (fibroblasts and endothelial cells). Both tumor proliferation and invasiveness were enhanced in the presence of stromal components. These results validate our 3D hydrogel model as a relevant platform to study cancer biology and tumor responses to biological treatments. (C) 2016 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据