期刊
BIOMATERIALS
卷 102, 期 -, 页码 268-276出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2016.06.022
关键词
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资金
- DOD [W911NF-09-1-0507]
- NIH [R21EB016359, R21CA174573]
- NSF [CMMI 1105539, CHE 1413666]
- Berkeley Chemical Biology Graduate Program (NIH) [1 T32 GMO66698]
- Croucher Foundation Scholarship
- Howard Hughes Medical Institute International Student Fellowship
- Direct For Mathematical & Physical Scien [1413666] Funding Source: National Science Foundation
- Directorate For Engineering
- Div Of Civil, Mechanical, & Manufact Inn [1105539] Funding Source: National Science Foundation
- Division Of Chemistry [1413666] Funding Source: National Science Foundation
Polyacrylamide hydrogels have been used extensively to study cell responses to the mechanical and biochemical properties of extracellular matrix substrates. A key step in fabricating these substrates is the conjugation of cell adhesion proteins to the polyacrylamide surfaces, which typically involves nonspecifically anchoring these proteins via side-chain functional groups. This can result in a loss of presentation control and altered bioactivity. Here, we describe a new functionalization strategy in which we anchor full-length extracellular matrix proteins to polyacrylamide substrates using 2-pyridinecarboxaldehyde, which can be co-polymerized into polyacrylamide gels and used to immobilize proteins by their N-termini. This one-step reaction proceeds under mild aqueous conditions and does not require additional reagents. We demonstrate that these substrates can readily conjugate to various extracellular matrix proteins, as well as promote cell adhesion and spreading. Notably, this chemistry supports the assembly and cellular remodeling of large collagen fibers, which is not observed using conventional side-chain amine-conjugation chemistry. (C) 2016 Elsevier Ltd. All rights reserved.
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