4.6 Article

The voltage-gated calcium channel CaV1.2 promotes adult oligodendrocyte progenitor cell survival in the mouse corpus callosum but not motor cortex

期刊

GLIA
卷 68, 期 2, 页码 376-392

出版社

WILEY
DOI: 10.1002/glia.23723

关键词

apoptosis; Cacna1C; calcium; CaV1.2; corpus callosum; motor cortex; NG2; oligodendrocyte; proliferation; survival; voltage-gated calcium channel

资金

  1. National Health and Medical Research Council [1066025, 1045240, 1139180]
  2. Ramaciotti Foundations [EQ2012/0007]
  3. Rebecca L Cooper Medical Research Foundation [Y0020971, P0024671]
  4. Royal Hobart Hospital Research Foundation [18-005]
  5. University of Tasmania
  6. MS Research Australia [17-0223]
  7. Macquarie Group Foundation [17-0223]
  8. Menzies Institute for Medical Research
  9. National Health and Medical Research Council of Australia [1139180, 1066025] Funding Source: NHMRC

向作者/读者索取更多资源

Throughout life, oligodendrocyte progenitor cells (OPCs) proliferate and differentiate into myelinating oligodendrocytes. OPCs express cell surface receptors and channels that allow them to detect and respond to neuronal activity, including voltage-gated calcium channel (VGCC)s. The major L-type VGCC expressed by developmental OPCs, CaV1.2, regulates their differentiation. However, it is unclear whether CaV1.2 similarly influences OPC behavior in the healthy adult central nervous system (CNS). To examine the role of CaV1.2 in adulthood, we conditionally deleted this channel from OPCs by administering tamoxifen to P60 Cacna1c(fl/fl) (control) and Pdgfr alpha-CreER:: Cacna1c(fl/fl) (CaV1.2-deleted) mice. Whole cell patch clamp analysis revealed that CaV1.2 deletion reduced L-type voltage-gated calcium entry into adult OPCs by similar to 60%, confirming that it remains the major L-type VGCC expressed by OPCs in adulthood. The conditional deletion of CaV1.2 from adult OPCs significantly increased their proliferation but did not affect the number of new oligodendrocytes produced or influence the length or number of internodes they elaborated. Unexpectedly, CaV1.2 deletion resulted in the dramatic loss of OPCs from the corpus callosum, such that 7 days after tamoxifen administration CaV1.2-deleted mice had an OPC density similar to 42% that of control mice. OPC density recovered within 2 weeks of CaV1.2 deletion, as the lost OPCs were replaced by surviving CaV1.2-deleted OPCs. As OPC density was not affected in the motor cortex or spinal cord, we conclude that calcium entry through CaV1.2 is a critical survival signal for a subpopulation of callosal OPCs but not for all OPCs in the mature CNS.

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