期刊
GENOME
卷 63, 期 2, 页码 103-114出版社
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/gen-2019-0071
关键词
DNA fingerprinting; SNP panel; identity analysis; Theobroma cacao accessions; genetic diversity
资金
- UWI-Trinidad and Tobago Research and Development Impact Fund
- International Fine Cocoa Innovation Centre (IFCIC)
- Cocoa Research Centre - European Union under the ACP Science & Technology Programme II
- University of the West Indies
u Single nucleotide polymorphisms (SNPs) are preferred markers for DNA fingerprinting and diversity studies in cacao (Theobroma cacao L.). Yet, a consensus SNP panel with a minimum number of SNPs for optimal identity analysis is unavailable for cacao. An initial set of 146 SNP panels of varying sizes were assembled based on heterozygosity, linkage disequilibrium (LD), linkage group (LG) distribution, major allele frequency, minor allele frequency (M(i)AF), polymorphism information content (PIC), and random distribution. These panels were assessed to determine their ability to distinguish among a training set of 155 accessions. The panels with the best separation ability were supplemented with additional SNPs to create 16 designer panels, which separated all 155 accessions. The 16 designer SNP panels were then assessed on a dataset of 1220 accessions coming from 10 ancestral groups. Increasing the number of SNPs generally yielded improved resolution of genetic identities with concomitant reduction of synonymous groups. The number and choice of SNPs were critical factors with LD, MiAF, and PIC being important selection attributes but an even LG distribution was unnecessary. A robust set of 96 SNPs is recommended as a minimal core SNP panel for cacao DNA fingerprinting to the international cacao community.
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