期刊
FUNGAL BIOLOGY
卷 125, 期 2, 页码 78-88出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.funbio.2019.11.007
关键词
Biological control; Actinobacteria; Fumonisins; Fungi; RNA-seq
类别
资金
- Agropolis Fondation [1403-026, ANR-10-LABX-0001-01]
- France Genomique National infrastructure
- Agence Nationale de la Recherche [ANR-10-INBS-09]
The study suggests that the actinobacteria Streptomyces sp. AV05 may be a potential biocontrol agent against mycotoxigenic fungi, specifically inhibiting the growth and mycotoxin production of F. verticillioides. RNA-seq data provided useful information on the genetic expression changes of both microorganisms during their interaction.
The actinobacteria Streptomyces sp. AV05 appears to be a potential biocontrol agent (BCA) against mycotoxigenic fungi. It was found to significantly inhibit F. verticillioides growth and mycotoxin production during their co-cultivation. F. verticillioides growth was durably affected while the decrease of the toxin production levels was reversible, suggesting different BCA actions. The study of both transcriptomes brought useful information on the microbial interaction. RNA-seq data indicated that the dual interaction modified genetic expression of both microorganisms as 18.5 % of the genes were differentially expressed for the fungus against 3.8 % for the actinobacteria. Fungal differentially expressed genes (DEGs) were equally up and down regulated while bacterial ones were mainly upregulated. We especially focused the analysis of DEGs on fungal defense reaction to bacterial attack. For example, if this potential BCA implements a strategy of antibiosis with the over expression of 'siderophore-interacting protein' linked to the production of bacteriocins, the fungus in a state of stress is able to adapt its metabolism by up-regulation of amidase. It could correspond to the induction of resistance gene clusters and suggest a detoxification process. Moreover fumonisins-related pathway appears underexpressed in the presence of Streptomyces that explain the reduction of fumonisin accumulation observed. (C) 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据