期刊
FOOD HYDROCOLLOIDS
卷 95, 期 -, 页码 195-202出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodhyd.2019.04.036
关键词
Starch microparticles; Amylopectin; Side chain elongation; Low digestibility; Amylosucrase; Pullulanase
资金
- National Key Research and Development Program of China [2017YFD0401103]
- national first-class discipline program of Food Science and Technology [JUFSTR20180203]
- National Natural Science Foundation of China (China) [31778198]
- University of Tennessee
- USDA NIFA Hatch Projects [TEN00487, 223984]
Enzymatically modified starch with controllable digestibility may find unique applications. In the present study, side chains of amylopectin were elongated by amylosucrase, followed by debranching with pullulanase to produce linear chains that self-assembled to form microparticles containing aggregated nanoparticles. Amylosucrase treatment for a longer time resulted in longer branches that were not changed after the subsequent modification by pullulanase. The A-type crystalline structure of native amylopectin was changed to the B-type and the percentage of crystallinity progressively increased after amylosucrase and subsequent pullulanase treatments. Elongation of branch chains favored the formation of thermal stable double helices, which decreased the contents of slowly digestible and rapidly digestible starches and increased the content of resistant starch from 9.1% of native amylopectin to be as much as 67.2% of microparticles. This study may provide an efficient and environmentally benign technology to produce modified starch with controllable physical and digestion properties enabling novel applications.
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