期刊
BIOMACROMOLECULES
卷 17, 期 2, 页码 615-621出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.biomac.5b01542
关键词
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资金
- U.S. Department of Energy under EPSCoR Grant [DE-SC0012432]
- Louisiana Board of Regents
- Russian Science Foundation [15-12-20021]
- GRO Fellowship Assistance Agreement U.S Environmental Protection Agency (EPA) [9177240]
- Russian Science Foundation [15-12-20021] Funding Source: Russian Science Foundation
Halloysite clay is an aluminosilicate nanotube formed by rolling flat sheets of kaolinite clay. They have a 15 nm lumen, 50-70 nm external diameter, length of 0.5-1 gm, and different inside/outside chemistry. Due to these nanoscale properties, they are used for loading, storage, and controlled release of active chemical agents, including anticorrosions, biocides, and drugs. We studied the immobilization in halloysite of laccase, glucose oxidase, and lipase. Overall, negatively charged proteins taken above their isoelectric points were mostly loaded into the positively charged tube's lumen. Typical tube loading with proteins was 6-7 wt % from which one-third was released in 5-10 h and the other two-thirds remained, providing enhanced biocatalysis in nanoconfined conditions. Immobilized lipase showed enhanced stability at acidic pH, and the optimum pH shifted to more alkaline pH. Immobilized laccase was more stable with respect to time, and immobilized glucose oxidase showed retention of enzymatic activity up to 70 degrees C, whereas the native sample was inactive.
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