期刊
FOOD BIOTECHNOLOGY
卷 33, 期 4, 页码 303-324出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/08905436.2019.1673171
关键词
Peptides; anti-inflammation; Synechococcus sp; VDW; nitric oxide; RAW 264; 7 macrophages
资金
- Grant for Research: Government Budget, Chulalongkorn University, Bangkok, Thailand [GRB_BSS_99_59_61_06]
- Ratchadaphiseksomphot Endowment Fund [R_016_2556]
- Center of Excellence on Medical Biotechnology (CEMB), S&T Postgraduate Education and Research Development Office (PERDO), Office of Higher Education Commission (OHEC), Thailand [SN-60-003-909]
The Synechococcus sp. VDW peptides were prepared by trypsin digestion, which was then purified by ultrafiltration with molecular weight cut off membranes of 10, 5 and 3 kDa. Among the fractions, the MW <3 kDa fraction exhibited high levels of inhibitory activity toward the NO radical scavenging activities with IC50 values of 34.51 ? 9.8 ?g/mL. The MW <3 kDa fraction was purified by reversed-phase HPLC to yield four fractions. The 30?40 min sub-fraction with maximum NO radical scavenging activity (77.50 ? 0.55%) was selected for further analysis by Q-TOF ESI mass spectrometry. Five isolated peptides with amino acid sequences of AILQSYSAGKTK; 1,265.69 Da, ALNKTHLIQTK; 1,265.74 Da, LLVHAPVK; 875.55 Da, IPDAHPVK; 875.48 Da, and VVVLRDGAVQQLGTPR; 1,706.97 Da were identified. The MW <3 kDa fraction showed no cytotoxicity toward RAW264.7 macrophage cells. Quantitative RT-PCR results showed that the MW <3 kDa fraction reduced gene expression of pro-inflammatory cytokines iNOS, TNF-?, COX-2, and IL-6. The peptides isolated from the MW <3 kDa fractions represent potential model peptides to develop natural anti-inflammation food-grade ingredients, drugs, and cosmetic products.
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