期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 133, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2019.110781
关键词
Raspberry; Oxidative damage; HepG2 cells; Keap1/Nrf2 pathway; Cyanidin 3-O-Glucoside
资金
- National Natural Science Foundation of China [31701520, 31801459]
- Funds for Distinguished Young Scientists from Fujian Agriculture and Forestry University [kxjq17012]
- Science and Technology Project of Fujian Province [2019J01393, 2019N0007]
The aim of the present study was to explore the protective effects of raspberry and its bioactive compound cyanidin 3-O-glucoside against H2O2-induced oxidative stress in HepG2 cells. We established a model of oxidative stress in HepG2 cells induced by H2O2 and examined the protein expression of Keap1/Nrf2. The antioxidant activity of raspberry extract was carried out measuring the level of reactive oxygen species (ROS), and the changes of phase II detoxification elements such as GSH level and CAT activity. Also the expression of proteins related to the Keap1/Nrf2 signaling was tested. The results revealed that raspberry extract significantly reduced the ROS levels in oxidative injured cells, increased GSH content and CAT activity, and activated the expression of proteins Keap1, Nrf2, HO-1, NQO1, and gamma-GCS. These results taken together indicated that raspberry treatment could ameliorate H2O2-induced oxidative stress in HepG2 cells via Keap1/Nrf2 pathway.
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