期刊
FISH & SHELLFISH IMMUNOLOGY
卷 94, 期 -, 页码 558-565出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2019.09.054
关键词
Aeromonas hydrophila; Mucin; Edwardsiella piscicida; Immunostimulant; Pectin; Spirulina maxima
资金
- Jeju Marine Research Institute, Korea Institute of Ocean Science and Technology (KIOST), Republic of Korea [PE99621, PE99722]
- Korea Institute of Marine Science & Technology Promotion (KIMST) [PE99722, PE99621] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
In this study, we demonstrate the enhanced disease resistance and positive immunomodulation of novel pectin isolated from Spirulina maxima (SmP) in zebrafish model. Zebrafish larvae exposed to SmP had significantly (p < 0.05) higher cumulative percent survival (CPS) at 25 (44.0%) and 50 mu g/mL (67.0%) against Edwardsieila piscicida compared to the control. However, upon Aeromonas hydrophila challenge, SmP exposed larvae at 50 mu g/mL had slightly higher CPS (33.3%) compared to control group (26.7%). SmP supplemented zebrafish exhibited the higher CPS against E. piscicida (93.3%) and A. hydrophila (60.0%) during the early stage of post-infection (< 18 hpi). qRT-PCR results demonstrated that exposing (larvae) and feeding (adults) of SmP, drive the modulation of a wide array of immune response genes. In SmP exposed larvae, up-regulation of the antimicrobial enzyme (lyz: 3.5-fold), mucin (muc5.1: 2.84, muc5.2: 2.11 and muc5.3: 2.40-fold), pro-inflammatory cytokines (il1 beta: 1.79-fold) and anti-oxidants (cat 2.87 and sod1: 1.82-fold) were identified. In SmP fed adult zebrafish (gut) showed > 2-fold induced pro-inflammatory cytokine (il1 beta) and chemokines (cxcl18b, ccl34a.4 and ccl34b.4). Overall results confirmed the positive modulation of innate immune responses in larval stage and it could be the main reason for developing disease resistance against E. piscicida and A. hydrophila. Thus, non-toxic, natural and biodegradable SmP could be considered as the potential immunomodulatory agent for sustainable aquaculture.
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