期刊
FISH & SHELLFISH IMMUNOLOGY
卷 93, 期 -, 页码 575-579出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2019.08.010
关键词
Silver catfish; Wound healing; beta-glucan; Cortisol; Histopathology
资金
- Secretaria de Desenvolvimento Economic, Ciencia e Tecnologia, Rio Grande do Sul, Brazil [SDECT 2438/2014, 338-25.00/14-0]
- Universidade de Passo Fundo Foundation (FUPF)
- CAPES
- FAPERGS
- CNPq [307900/2016-9]
The immune modulating activity of beta-glucan on aquatic species has been a matter of intense investigation. Here, we aimed to investigate the effect of beta-glucan on wound healing of silver catfish, a Neotropical South American scale-free fish. Small sections of skin and muscle (3 mm in diameter) were removed and fish were bathed daily with beta-glucan (0.1% and 0.5%) up to 28 days when cicatrization was complete. A group of fish similarly injured and non-exposed to beta-glucan was used as control. Wound closure and healing was monitored visually and by histopathological analysis. In fish bathed with 0.5% beta-glucan we found reduced blood cortisol levels at day one post-wounding and, by day 7 post wounding, the deposition of granulation tissue was higher compared to non-exposed fish. In addition, from day 7 forward, wound size was significantly lower in fish bathed with 0.5% beta-glucan. Histopathological analysis of the wounded site indicated a thin layer of immature epidermal cells at day one post wounding. A discrete inflammation with mixed inflammatory cell infiltrate was observed on wounded muscle and was lower by day 7 post wounding on fish bathed with 0.5% beta-glucan. By day 14 post wounding, the deposition of collagen fibers and the presence of fibroblast and new muscle fibers were higher in fish exposed to 0.5% beta-glucan, and dermis restoration was complete. Thus, our results indicate that in silver catfish wound healing occurs rapidly and improves greatly by daily bathing with beta-glucan.
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