期刊
BIOMACROMOLECULES
卷 17, 期 12, 页码 3893-3901出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.biomac.6b01242
关键词
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资金
- NanoNextNL, a micro and nanotechnology consortium of the government of The Netherlands
- ERC [267254]
- European Research Council (ERC) [267254] Funding Source: European Research Council (ERC)
Previously, we developed triblock protein polymers that form fibrilar hydrogels at low protein polymer concentrations (denoted C-2-S-48(H)-C-2). We here demonstrate that the structure of these hydrogels can be tuned via heterodimeric coiled coils that cross-link and bundle the self-assembled protein polymer fibrils. We fused well-characterized, 47 amino acids-long heterodimeric coiled coil linkers (D-A or D-B) to the C-terminus of the triblock polymer. The resulting C-2-S-48(H)-C-2-D-A and C-2-S-48(H)-C-2-D-B polymers, were successfully produced as secreted proteins in Pichia pastoris, with titers of purified protein in the order of g L-1 of clarified broth. Atomic force microscopy showed that fibrils formed by either C-2-S-48(H)-C-2-D-A or C-2-S-48(H)-C-2-D-B alone already displayed extensive bundling, apparently as a result of homotypic (D-A/D-A and D-B/D-B) interactions. For fibrils prepared from protein polymers having no linkers, plus a small fraction of polymers containing either D-A or D-B linkers, no cross-linking and bundling was observed. At these same low concentrations of linkers, fibrils containing both the D-A and the D-B linkers did show cross-linking and bundling as a consequence of heterodimer formation. This work shows that we can control the extent of bundling and cross-linking of supramolecular fibrils by varying the density of heterodimerizing coiled coils in the fibrils, which is promising for the further development of materials that mimic the extracellular matrix.
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