期刊
BIOMACROMOLECULES
卷 17, 期 4, 页码 1383-1394出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.biomac.6b00005
关键词
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资金
- VINNOVA, Mobility for Growth [201304323]
- Marie Curie Actions FP7-PEOPLE-2011-CO-FUND [GROWTH 291795]
- Swedish Research Council [621-2013-3764]
Biodegradable aliphatic polyesters such as poly(lactide) and poly(epsilon-caprolactone), largely used in tissue engineering applications, lack suitable functional groups and biological cues to enable interactions with cells. Because of the ubiquity of thiol groups in the biological environment and the pliability of thiol chemistry, we aimed to design and synthesize poly(ester) chains bearing pendant thiol-protected groups. To achieve this, 3-methyl-6-(tritylthiomethyl)-1,4-dioxane-2,5-dione, a lactide-type monomer possessing a pendant thiol-protected group, was synthesized. This molecule, when used as a monomer in controlled ring-opening polymerization in combination with lactide and epsilon-caprolactone, appeared to be a convenient building block for the preparation of functionalized aliphatic copolyesters, which were easily modified further. A polymeric sample bearing pyridyl disulfide groups, able to bind a cysteine-containing peptide, was efficiently obtained from a two-step modification reaction. Porous scaffolds were then prepared by blending this latter copolymer sample with poly(L-lactide-co-epsilon-caprolactone) followed by salt leaching. A further disulfide exchange reaction performed in aqueous medium formed porous scaffolds with covalently linked arginine-glycine-aspartic acid sequences. The scaffolds were characterized by thermal and mechanical tests, and scanning electron microscopy surface images revealed a highly porous morphology. Moreover, a cytotoxicity test indicated good cell viability.
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