期刊
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
卷 2019, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2019/6497691
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资金
- Korea Institute of Oriental Medicine - Ministry of Education, Science and Technology (MEST), Republic of Korea [KSN1812102]
- Ministry of Oceans and Fisheries, Republic of Korea [20190055]
- National Research Council of Science & Technology (NST), Republic of Korea [KSN1812102] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Rhus verniciflua is widely known for its antioxidant, antibacterial, anticancer, and antiaging efficacy and alpha-glucosidase inhibition. This study was designed whether Rhus verniciflua extracts inhibit the IgE-antigen-mediated allergic reaction in RBL-2H3 mast cells, and it further investigated the Fc epsilon RI- and arachidonate-signaling by which Rhus verniciflua extracts exert its antiallergic effects. IgE-antigen-sensitized RBL-2H3 mast cells were investigated for the cytotoxicity of Rhus verniciflua extracts and beta-hexosaminidase release, and inflammatory mediators (e.g., TNF-alpha, IL-4, IL-6, histamine, and PGD(2)) were then assessed. Additionally, we examined expressions of genes involved in arachidonate- and Fc epsilon RI-signaling pathway in RBL-2H3. Rhus verniciflua extracts inhibited beta-hexosaminidase release and production of the inflammatory mediators in RBL-2H3. Rhus verniciflua extracts reduced amounts of histamine and expressions of Fc epsilon RI signaling-related genes such as Lyn and Syk and phosphorylation of extracellular signal-regulated kinase in mast cells. Finally, in late allergic responses, Rhus verniciflua extracts reduced PGD(2) release and COX-2 and cPLA2 phosphorylation expressions from IgE-antigen-mediated mast cells. Lastly, 250-500 mg/kg RVE significantly attenuated the Ag/IgE-induced passive cutaneous anaphylaxis (PCA) reaction in mice. These findings provide novel information on the molecular mechanisms underlying the antiallergy properties of Rhus verniciflua extracts in Fc epsilon RI-mediated allergic reaction.
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