期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 50, 期 1, 页码 56-62出版社
WILEY
DOI: 10.1002/eji.201948151
关键词
deoxyguanosine; guanosine; R848; ssRNA; TLR7
类别
资金
- UK Medical Research Council [MRC core funding of the MRC Human Immunology Unit]
- Wellcome Trust [100954]
- Wellcome Trust Infection, Immunology & Translational Medicine doctoral programme [105400/Z/14/Z]
- MRC [MC_UU_00008/8] Funding Source: UKRI
- Wellcome Trust [105400/Z/14/Z] Funding Source: Wellcome Trust
Toll-like receptor 7 (TLR7) is an innate immune sensor for single-strand RNA (ssRNA). Recent structural analysis revealed that TLR7 has an additional binding site for nucleosides such as guanosine, and is activated when both guanosine and ssRNA bind. The nucleoside binding site also accommodates imidazoquinoline derivatives such as R848, which activate TLR7 in the absence of ssRNA. Here, we report that deoxyguanosine (dG) triggered cytokine production in murine bone marrow derived macrophages and plasmacytoid dendritic cells, as well as in human peripheral blood mononuclear cells, including type I interferons and pro-inflammatory factors such as TNF and IL-6. This signalling activity of dG was dependent on TLR7 and its adaptor MyD88 and did not require amplification via the type I interferon receptor. dG-triggered cytokine production required endosomal maturation but did not depend on the concurrent provision of RNA. We conclude that dG induces an inflammatory response through TLR7 and propose that dG is an RNA-independent TLR7 agonist.
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