期刊
EUROPEAN JOURNAL OF HISTOCHEMISTRY
卷 63, 期 4, 页码 204-213出版社
PAGEPRESS PUBL
DOI: 10.4081/ejh.2019.3059
关键词
Innervation; lymph node; dendritic cell; lymphocytes; neurofilament; immunofluorescence staining
类别
The peripheral nervous system communicates specifically with the immune system via local interactions. These interactions include the hardwiring of sympathetic/parasympathetic (efferent) and sensory nerves (afferent) to primary (e.g., thymus and bone marrow) and secondary (e.g., lymph node, spleen, and gut-associated lymphoid tissue) lymphoid tissue/organs. To gain a better understanding of this bidirectional interaction/crosstalk between the two systems, we have investigated the distribution of nerve fibres and PNS-immune cell associations in situ in the mouse lymph node by using immunofluorescent staining and con focal microscopy/ three-dimensional reconstruction. Our results demonstrate: i) the presence of extensive nerve fibres in all compartments (including B cell follicles) in the mouse lymph node; ii) close contacts/associations of nerve fibres with blood vessels (including high endothelial venules) and lymphatic vessels/sinuses; close contacts/associations of nerve fibres with various subsets of dendritic cells (e.g., B220(+)CD11c(+), CD4(+)CD11c(+) , CD8a(+). CD 11c(+), and Mac1(+)CD 11c(+)), Mac 1(+) macrophages, and B/T lymphocytes. Our novel findings concerning the innervation and nerve-immune cell interactions inside the mouse lymph node should greatly facilitate our understanding of the effects that the peripheral nervous system has on cellular- and humoral-mediated immune responses or vice versa in health and disease.
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