期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 130, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2019.109371
关键词
Bradyrhizobium diazoefficiens; Methanol utilization; Methanol dehydrogenase; Lanthanide, XoxF
资金
- Joint Usage/Research Center, Institute of Plant Science and Resources, Okayama University, Japan
- [17H03949]
The legume symbiotic nitrogen-fixing bacterium, B. diazoefficiens strain USDA110, utilizes methanol for growth in the presence of light lanthanides, such as La3+, Ce3+, Pr3+ or Nd3+, and its cells possess significant methanol dehydrogenase (MDH) activity. We purified MDH to homogeneity from B. diazoefficiens strain USDA110 grown in a methanol/Ce3+ medium; the protein was identified as XoxF5-type MDH (blr6213). The purified XoxF contained 0.58 cerium atoms per enzyme subunit. Moreover, the in-solution structure of XoxF was analyzed by small angle X-ray scattering (SAXS) analysis; the radius of gyration (R-8) and maximum particle dimension (D-max) of XoxF were calculated to be 32.3 and 96.8 angstrom, respectively, suggesting that XoxF adopts a dimer structure in solution. These results show that B. diazoefficiens strain USDA110 has XoxF, a lanthanides-dependent MDH, which has methanol oxidation activity and is induced by methanol/lanthanaides, and that lanthanide is one of the important factors in methanol utilization by the strain.
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