期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 130, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2019.109360
关键词
Anti-Str. uberis antibodies; Bioinformatic analysis; Affinity; Immunodetection of Sir. uberis; Milk analysis
资金
- Estonian Research Council [IUT 20-17]
- Estonian Dairy Cluster - Estonian Rural Development Plan2015-2020
- European Agricultural Fund for Rural Development
Streptococcus uberis is a major mastitis-causing environmental pathogen, which rapid immunodetection has not been possible due to the absence of specific anti-Str. uberis antibodies. Recently, a specific antibody against the Str. uberis adhesion molecule (SUAM) has been designed. In the present study, the specificity and affinity of this antibody towards SUAM antigenic region SAPVYLGVSTE and Str. uberis cells are characterized, using experimental and in silico bioinformatic methods. The selectivity studies and bioinformatic analyses revealed high specificity of the antibody towards Str. uberis. The K-d value of SAPVYLGVSTE/anti-Str. uberis antibody complex was 27 +/- 6 nM, indicating the applicability of this antibody for the detection of Str. uberis. The anti-Sir. uberis antibody was used as a specific biorecognition element of a biosensor for the detection of Str. uberis bacteria in phosphate buffer and in milk and these analyses took less than 20 min. The Sir. uberis biosensor was also tested in the milk of cows suffering from mastitis and the obtained results were in good agreement with the conventional identification of this pathogen by microbiological plating.
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