期刊
ENVIRONMENTAL POLLUTION
卷 257, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2019.113517
关键词
MBP; Zebrafish; Liver; Oxidative stress; Apoptosis
资金
- Second Batch of National 10,000 Person Plan in 2016
- Plan for Training Talents with Special Funds for the Development of Local Colleges and Universities by Central Finance
- National Natural Science Foundation of China [41877128]
- National Natural Science Fund for Distinguished Young Scholars [41625002]
- MOA Modern Agricultural Talents Support Project
- Young Talents Project of Northeast Agricultural University [18QC13]
- Heilongjiang Provincial Key Laboratory of Soil Protection and Remediation
In this paper, the acute toxicity of monobutyl phthalate (MBP), the main hydrolysis product of dibutyl phthalate, on adult zebrafish liver antioxidant system was studied. Compared the toxicity effect of MBP and DBP by histopathology and apoptosis experiments, we speculated that the toxic effects of DBP on animals may be caused by its metabolite MBP. The results indicated that the antioxidant Nrf2-Keap1 pathway was insufficient to resist MBP-induced hepatotoxicity and led to an imbalance of membrane ion homeostasis and liver damage. Decreased cell viability, significant tissue lesions and early hepatocyte apoptosis were observed in the zebrafish liver in MBP exposure at high concentration (10 mg/L). The activities of antioxidant enzymes and ATPases in zebrafish liver were inhibited with increased malondialdehyde (MDA) content and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities. Integrated biomarker response (IBR) calculation results indicated that MBP mainly inhibited catalase (CAT) activity. Simultaneously, the expression of antioxidant-related genes (SOD, CAT, GPx, Nrf2, HO-1) was down-regulated, while apoptosis-related genes (p53, box, cas3) were significantly upregulated. (C) 2019 Elsevier Ltd. All rights reserved.
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