Effects of different metal ions (Ca, Cu, Pb, Cd) on formation of cyanobacterial blooms

Eastern China is a typical region that suffers from harmful cyanobacterial blooms. Numerous studies have focused on bloom formation mechanisms; however, the detailed mechanisms remained unclear. Our study explored the influence of four metal ions (Ca, Cu, Pb and Cd) on Microcystis aeruginosa to determine their effects on bloom formation. We found that Ca concentrations higher than 100 mg L-1 contributed to cyanobacterial bloom formation. The presence of Ca triggered the anti-oxidation process and promoted the secretion of extracellular polysaccharides, thus inducing aggregation of algal cells and enhancing their buoyancy 2.1-fold more than the control (p < 0.05). The reverse regulation of dissolved CO2 to bicarbonate by carbonic anhydrase formed a large amount of carbonate and decreased the growth rate by 38-56%. Cu (> 0.1 mg L-1) presented significant toxicity to algal cells while Pb (> 1 mg L-1) suppressed the algal growth rate due to the acidic condition. Cd (< 0.1 mg L-1) exhibited no apparent toxicity to the algae. Furthermore, as the buoyancy increased, Cd was likely to facilitate the formation of cyanobacterial blooms, which needs further research. These findings can provide a theoretical basis for eutrophic lake management and contribute to the development of water quality and wastewater discharge standards.