期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 182, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2019.109473
关键词
Clenbuterol; Monoclonal antibody; Immunoassay; Open sandwich ELISA
资金
- National Natural Science Foundation of China [21775064]
- Shandong Provincial Natural Science Foundation [ZR2017MB037]
- JSPS KAKENHI from the Japan Society for the Promotion of Science, Japan [JP18H03851]
- Dynamic Alliance for Open Innovation Bridging Human, Environment and Materials from MEXT, Japan
Clenbuterol (CLEN) is a sympathomimetic amine used as a decongestant and bronchodilator while treating breathing disorders. It is also used in food-producing animals as it improves the rate of red meat production. However, it is prohibited in many countries nowadays due to human health and safety concerns. Unfortunately, the illegal use of CLEN is still rampant. Thus, monitoring it in food and livestock is important. Here, we report a novel murine antibody and an open sandwich enzyme linked immunosorbent assay (OS-ELISA) to detect CLEN based on antigen-antibody reactions. The genes of antibody variable regions in mice immunized with CLEN conjugated with bovine serum albumin were cloned into a phagemid (pDong1/Fab) to construct a phage-display antibody library, from which a novel antibody, A12, was selected. Then, an OS-ELISA was developed to detect CLEN using separated variable regions of the A12 antibody. The limit of detection of the assay was found to be 8 ng/mL, which was useful for monitoring CLEN usage.
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