4.7 Article

Three-dimensional live imaging of Atoh1 reveals the dynamics of hair cell induction and organization in the developing cochlea

期刊

DEVELOPMENT
卷 146, 期 21, 页码 -

出版社

COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.177881

关键词

Atoh1; Cochlea; Hair cell; Live imaging; Mouse

资金

  1. Core Research for Evolutional Science and Technology (CREST) [JPMJCR12W2]
  2. Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan [16H06480, 25462635]
  3. Program for Technological Innovation of Regenerative Medicine from the Japan Agency for Medical Research and Development [JP18bm0704020]
  4. Takeda Medical Research Foundation
  5. Grants-in-Aid for Scientific Research [25462635] Funding Source: KAKEN

向作者/读者索取更多资源

During cochlear development, hair cells (HCs) and supporting cells differentiate in the prosensory domain to form the organ of Corti, but how one row of inner HCs (IHCs) and three rows of outer HCs (OHCs) are organized is not well understood. Here, we investigated the process of HC induction by monitoring Atoh1 expression in cochlear explants of Atoh1-EGFP knock-in mouse embryos and showed that only the cells that express Atoh1 over a certain threshold are selected for HC fate determination. HC induction initially occurs at the medial edge of the prosensory domain to form IHCs and subsequently at the lateral edge to form OHCs, while Hedgehog signaling maintains a space between IHCs and OHCs, leading to formation of the tunnel of Corti. These results reveal dynamic Atoh1 expression in HC fate control and suggest that multi-directional signals regulate OHC induction, thereby organizing the prototype of the organ of Corti.

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