期刊
DEVELOPMENT
卷 146, 期 22, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.183053
关键词
Drosophila; Male meiosis; Centrosome; Cytokinesis; Spermatogenesis
资金
- Associazione Italiana per la Ricerca sul Cancro [IG2017 20779]
- National Institutes of Health [R01GM124054, R01GM62276]
- Federazione Italiana per la Ricerca sul Cancro-Associazione Italiana per la Ricerca sul Cancro [19686]
During the extended prophase of Drosophila gametogenesis, spermatocytes undergo robust gene transcription and store many transcripts in the cytoplasm in a repressed state, until translational activation of select mRNAs in later steps of spermatogenesis. Here, we characterize the Drosophila Doublefault (Dbf) protein as a C2H2 zinc-finger protein, primarily expressed in testes, that is required for normal meiotic division and spermiogenesis. Loss of Dbf causes premature centriole disengagement and affects spindle structure, chromosome segregation and cytokinesis. We show that Dbf interacts with the RNA-binding protein Syncrip/hnRNPQ, a key regulator of localized translation in Drosophila. We propose that the pleiotropic effects of dbf loss-of-function mutants are associated with the requirement of dbf function for translation of specific transcripts in spermatocytes. In agreement with this hypothesis, Dbf protein binds cyclin B mRNA and is essential for translation of cyclin B in mature spermatocytes.
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