期刊
CURRENT BIOLOGY
卷 29, 期 22, 页码 3791-+出版社
CELL PRESS
DOI: 10.1016/j.cub.2019.09.024
关键词
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资金
- Ministerio de Economia y Competitividad, Spain [BFU2015-65501-P]
- FEDER [RYC-2014-15844]
- Ministerio de Ciencia, Innovacion y Universidades, Spain [PGC2018-097452-B-I00]
- (FEDER)
- Ministerio de Educacion, Cultura y Deporte, Spain [FPU14/01983, FPU14/01832]
- Tata Innovation Fellowship, Department of Biotechnology, Government of India [BT/HRD/35/01/03/2017]
- DBT-BUILDER programme [BT/INF22/SP27679/2018]
- JNCASR
- DST-WOS-A postdoctoral fellowship [SR/WOS-A/LS-207/2018]
- NIH/NIAID [R37, AI39115-21]
- R01 grant [AI50113-15]
- US Department of Energy Joint Genome Institute, a DOE Office of Science User Facility
- Office of Science of the US Department of Energy [DE-AC02-05CH11231]
Centromeres are rapidly evolving across eukaryotes, despite performing a conserved function to ensure high-fidelity chromosome segregation. CENP-A chromatin is a hallmark of a functional centromere in most organisms. Due to its critical role in kinetochore architecture, the loss of CENP-A is tolerated in only a few organisms, many of which possess holocentric chromosomes. Here, we characterize the consequence of the loss of CENP-A in the fungal kingdom. Mucor circinelloides, an opportunistic human pathogen, lacks CENP-A along with the evolutionarily conserved CENP-C but assembles a monocentric chromosome with a localized kinetochore complex throughout the cell cycle. Mis12 and Dsn1, two conserved kinetochore proteins, were found to co-localize to a short region, one in each of nine large scaffolds, composed of an similar to 200-bp AT-rich sequence followed by a centromere-specific conserved motif that echoes the structure of budding yeast point centromeres. Resembling fungal regional centromeres, these core centromere regions are embedded in large genomic expanses devoid of genes yet marked by Grem-LINE1s, a novel retrotransposable element silenced by the Dicerdependent RNAi pathway. Our results suggest that these hybrid features of point and regional centromeres arose from the absence of CENP-A, thus defining novel mosaic centromeres in this earlydiverging fungus.
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