期刊
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
卷 47, 期 3, 页码 517-519出版社
WILEY
DOI: 10.1111/1440-1681.13209
关键词
calcium-sensing receptor; osteosarcoma cells; proliferation; signalling pathway
资金
- Young and Middle-aged Innovation Fund of the Second Affiliated Hospital of Harbin Medical University [KYCX 2018-07]
- Natural Science Foundation of Heilongjiang [H2015105]
Our results showed that the expression of calcium-sensing receptor (CaSR) and the concentration of intracellular calcium were enhanced in the osteosarcoma cells MG-63 compared with in the normal osteoblasts hFOB1.19. GdCl3 (CaSR agonist) significantly increased the proliferation rate of MG-63 cells, the expression of PCNA and Cyclin D1 and decreased the expression of p21(Cip/WAF-1). The effect of NPS2390 (CaSR antagonist) on the above indicators was opposite to GdCl3. In addition, GdCl3 up-regulated the phosphorylation of ERK1/2, PI3K and Akt and the proliferation rate of MG-63 cells. However, these effects of GdCl3 were cancelled by LY294002 (a PI3K inhibitor) or PD98059 (an ERK1/2 inhibitor). Our results demonstrate that activation of CaSR promotes osteosarcoma cell multiplication by up-regulating ERK1/2 and PI3K-Akt pathways.
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