期刊
CLINICAL & TRANSLATIONAL ONCOLOGY
卷 22, 期 7, 页码 1013-1022出版社
SPRINGER INT PUBL AG
DOI: 10.1007/s12094-019-02225-5
关键词
Baicalein (BE); Baicalin (BI); Scutellaria baicalensis; Intestinal microbiota; Apc(Min); + mouse model; Anti-inflammation; Antitumor; Colorectal cancer
类别
资金
- NIH/NCCAM [P01 AT004418, K01 AT005362]
- Chinese Grants from the 111 Project from the Ministry of Education of China
- State Administration of Foreign Experts Affairs of China [B16046]
- National Natural Science Foundation of China [81603378]
- Natural Science Foundation of Jiangsu Province [BK20160545]
Objective Chronic inflammation is recognized as a risk factor for colorectal cancer (CRC) development. Baicalin (BI), a major constituent in an anti-inflammatory herb Scutellaria baicalensis, can be biotransformed into baicalein (BE) by the intestinal microbiota. We evaluated the anti-inflammation and anti-CRC effects of the metabolite BE. Methods The in vitro biotransformation by human intestinal microbiota from BI into BE has been determined with HPLC. Using a gut-specific Apc(Min/+) mouse model, the effects of oral BE on the life span, organ index, and tumor multiplicity were evaluated. The expressions of inflammatory cytokines were determined using ELISA. To verify the in vivo data, the anti-inflammatory and antiproliferative effects of BE were determined with an in vitro cell model. Results HPLC analysis showed that BI was quickly transformed into BE by the intestinal microbiota. Oral BE (30 mg/kg/day) significantly increased the life span, from 125.2 to 218.4 days (P < 0.01%). BE treatment also decreased intestine index and increased spleen index. Compared with the model group, following BE treatment, tumor numbers were significantly reduced in the small intestine and colon (P < 0.01, P < 0.05, respectively). In the gut tissues, BE treatment significantly reduced inflammatory cytokine levels such as IL-1 beta, IL-2, IL-6, IL-10, G-CSF, and GM-CSF. In vitro data supported our in vivo results that the anti-CRC effects of BE were via the inhibition of gut inflammation and induction of cancer cell death. Conclusion Our results suggest that the parent compound BI can be quickly converted into its microbial metabolite BE, which has stronger bioactive effects than BI. Baicalein is an active chemopreventive metabolite for inflammatory associated CRC.
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